Coyne S I, Mendelson N H
Infect Immun. 1975 Nov;12(5):1189-94. doi: 10.1128/iai.12.5.1189-1194.1975.
Purified minicells produced by Bacillus subtilis div IV-B1 mutants were used to immunize rabbits. Immune serum was obtained that agglutinated minicells and was able to form precipitin lines when reacted with minicell antigens in double-diffusion or immunoelectrophoretic procedures. Antiminicell serum agglutinated rod-shaped B. subtilis cells to long filaments produced by growth of a cell division-defective mutant at restrictive temperature. These findings indicate that minicells are immunogens capable of eliciting the production of antibodies that cross-react with the lateral, cylindrical regions of B. subtilis rods. It appears, therefore, that poles share a common antigen(s) with cylindrical regions of the cell.
由枯草芽孢杆菌div IV - B1突变体产生的纯化微细胞被用于免疫兔子。获得了免疫血清，该血清能凝集微细胞，并且在双扩散或免疫电泳程序中与微细胞抗原反应时能够形成沉淀线。抗微细胞血清将杆状枯草芽孢杆菌细胞凝集为细胞分裂缺陷型突变体在限制温度下生长产生的长丝。这些发现表明微细胞是能够引发产生与枯草芽孢杆菌杆状菌的侧面、圆柱形区域发生交叉反应的抗体的免疫原。因此，似乎细胞的极与圆柱形区域共享一种或多种共同抗原。
