Hoshino Y, Nishikawa K, Benfield D A, Gorziglia M
Epidemiology Section, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892.
Virology. 1994 Feb 15;199(1):233-7. doi: 10.1006/viro.1994.1117.
Two neutralizing monoclonal antibodies (N-mAbs) were utilized to locate amino acid (aa) residues involved in the formation of serotype-cross-reactive epitopes on the VP7 of selected group A rotaviruses. N-mAb 954/159/13 neutralized G serotype 3 as well as porcine G serotype 4 rotaviruses, whereas N-mAb 57/8 neutralized G serotype 3, 4, 6, 9, and 10 strains. Neutralization-resistant variants of each serotype were selected in the presence of these two monoclonal antibodies. Sequence analysis of the gene encoding VP7 of such variants revealed: (i) variable regions VR-5 (aa 88-100), VR-8 (aa 209-223), and VR-9 (aa 235-242) are involved in cross-reactive neutralization; (ii) an aa substitution can occur at the same position on the VP7 of different serotypes selected by a given N-mAb; (iii) the location of an aa substitution on the variant VP7 selected by a given N-mAb can vary depending on the rotavirus serotype; (iv) a substituted single aa species at a specific position on the variant VP7 selected by a single N-mAb can vary, resulting in variants which exhibit antigenic differences; and (v) the VP7 of a porcine rotavirus Gottfried strain has a unique antigenic mosaic of serotype 3 and serotype 4.
利用两种中和单克隆抗体（N - mAbs）来定位选定的A组轮状病毒VP7上参与形成血清型交叉反应表位的氨基酸（aa）残基。N - mAb 954/159/13可中和G血清型3以及猪G血清型4轮状病毒，而N - mAb 57/8可中和G血清型3、4、6、9和10毒株。在这两种单克隆抗体存在的情况下，选择了每种血清型的中和抗性变体。对这些变体的VP7编码基因进行序列分析发现：（i）可变区VR - 5（氨基酸88 - 100）、VR - 8（氨基酸209 - 223）和VR - 9（氨基酸235 - 242）参与交叉反应中和；（ii）在由给定N - mAb选择的不同血清型的VP7上，同一位置可能发生氨基酸替代；（iii）由给定N - mAb选择的变体VP7上氨基酸替代的位置可能因轮状病毒血清型而异；（iv）由单个N - mAb选择的变体VP7上特定位置的单个替代氨基酸种类可能不同，从而产生表现出抗原差异的变体；（v）猪轮状病毒Gottfried毒株的VP7具有血清型3和血清型4独特的抗原嵌合体。
