Laserson K F, Petralanda I, Hamlin D M, Almera R, Fuentes M, Carrasquel A, Barker R H
Department of Population and International Health, Harvard School of Public Health, Boston, Massachusetts.
Am J Trop Med Hyg. 1994 Feb;50(2):169-80. doi: 10.4269/ajtmh.1994.50.169.
We have examined the reproducibility, sensitivity, and specificity of detecting Plasmodium falciparum using the polymerase chain reaction (PCR) and the species-specific probe pPF14 under field conditions in the Venezuelan Amazon. Up to eight samples were field collected from each of 48 consenting Amerindians presenting with symptoms of malaria. Sample processing and analysis was performed at the Centro Amazonico para la Investigacion y Control de Enfermedades Tropicales Simon Bolivar. A total of 229 samples from 48 patients were analyzed by PCR methods using four different P. falciparum-specific probes. One P. vivax-specific probe and by conventional microscopy. Samples in which results from PCR and microscopy differed were reanalyzed at a higher sensitivity by microscopy. Results suggest that microscopy-negative, PCR-positive samples are true positives, and that microscopy-positive and PCR-negative samples are true negatives. The sensitivity of the DNA probe/PCR method was 78% and its specificity was 97%. The positive predictive value of the PCR method was 88%, and the negative predictive value was 95%. Through the analysis of multiple blood samples from each individual, the DNA probe/PCR methodology was found to have an inherent reproducibility that was highly statistically significant.
我们在委内瑞拉亚马逊地区的野外条件下，使用聚合酶链反应（PCR）和物种特异性探针pPF14检测恶性疟原虫，检验了其重现性、敏感性和特异性。从48名有疟疾症状的同意参与的美洲印第安人身上，每人最多野外采集8份样本。样本处理和分析在西蒙·玻利瓦尔热带疾病研究与控制亚马逊中心进行。使用四种不同的恶性疟原虫特异性探针，通过PCR方法对48名患者的总共229份样本进行了分析。使用一种间日疟原虫特异性探针，并通过传统显微镜检查。PCR和显微镜检查结果不同的样本，通过显微镜以更高的敏感性重新分析。结果表明，显微镜检查阴性、PCR阳性的样本为真阳性，显微镜检查阳性、PCR阴性的样本为真阴性。DNA探针/PCR方法的敏感性为78%，特异性为97%。PCR方法的阳性预测值为88%，阴性预测值为95%。通过分析每个个体的多个血液样本，发现DNA探针/PCR方法具有高度统计学意义的内在重现性。
