Jung J S, Cheong T C, Cho M S, Hah Y C, Chung J H
Department of Biology, Sunchon National University, Korea.
Biochem Biophys Res Commun. 1994 Feb 15;198(3):1084-9. doi: 10.1006/bbrc.1994.1154.
The 1.0 kb fragment carrying a gentamicin resistance determinant was subcloned from the R plasmid in clinical isolates of Serratia marcescens and its nucleotide sequence was determined. It contains an open reading frame composed of 858 nucleotides, which shows 97% homology to the aacC2 gene in nucleotide sequence. Minicells carrying the 1.0 kb insert DNA on the plasmids produced a 30 kd protein that was consistent with the expected size of the deduced amino acid sequence from the open reading frame. A search for the promoter revealed a putative promoter of which the -35 region was provided by the right end of Tn3, suggesting that the expression of the gentamicin resistance gene is dependent on the Tn3 sequence.
携带庆大霉素抗性决定簇的1.0 kb片段从粘质沙雷氏菌临床分离株的R质粒中进行亚克隆，并测定了其核苷酸序列。它包含一个由858个核苷酸组成的开放阅读框，其核苷酸序列与aacC2基因显示出97%的同源性。在质粒上携带1.0 kb插入DNA的微小细胞产生了一种30 kd的蛋白质，这与从开放阅读框推导的氨基酸序列的预期大小一致。对启动子的搜索揭示了一个推定的启动子，其-35区域由Tn3的右端提供，这表明庆大霉素抗性基因的表达依赖于Tn3序列。
