Gaidamakova E K, Karginov V A, Kovalenko S P
Institute of Molecular Pathology and Ecological Biochemistry, Novosibirsk, Russia.
Biochem Biophys Res Commun. 1994 Feb 15;198(3):862-8. doi: 10.1006/bbrc.1994.1123.
Protein-binding DNA-regions inside and in the vicinity of the phenobarbital-inducible P450bm-3 gene from Bacillus megaterium were characterized by gel-retardation technique and foot-printing analysis. Regions with induction-dependent protein binding capacity were shown to be situated in -279bp/-215bp, -215bp/+83bp and +236bp/+425bp fragments. Precise localization of DNA-protein contacts was established by foot-printing analysis for region -215bp/+83bp. The data obtained are discussed in line with previously reported information on the regulatory regions of various phenobarbital-regulated genes.
采用凝胶阻滞技术和足迹分析对巨大芽孢杆菌中苯巴比妥诱导型P450bm - 3基因内部及附近的蛋白质结合DNA区域进行了表征。结果表明，具有诱导依赖性蛋白质结合能力的区域位于-279bp/-215bp、-215bp/+83bp和+236bp/+425bp片段中。通过足迹分析确定了-215bp/+83bp区域中DNA - 蛋白质接触的精确位置。结合先前报道的各种苯巴比妥调节基因调控区域的信息，对所获得的数据进行了讨论。
