人类三克隆抗IgG丙种球蛋白病。III. 对IgM自身抗体四年半的克隆持续性的测定。
Human triclonal anti-IgG gammopathy. III. Determination of the clonal persistence of the IgM autoantibody for a four and one-half year period.
作者信息
Abraham G N, Brown P, Johnston S L, Nellis L, Marks S, Welch E H
出版信息
Immunology. 1978 Sep;35(3):447-53.
The persistence in the structure of IgM anti-IgGs obtained from the serum of a patient (Gil) with Felty's syndrome was studied. Co-isoelectric focusing experiments showed that intact IgM from early (1970) and late (1973) sera, and their heavy and light chains had identical isoelectric points (pI). Light chains from 1970, 1973, and 1974 IgM-Gil had identical amino terminal amino acid sequences and belonged to the v Kappa II light chain subgroup. Specificity studies of IgM-Gil isolated from serum obtained in 1970, 1972 and 1974 showed identical patterns of reactivity with IgG and IgG subunits of all gamma chain subclasses. Finally, by haemagglutination-inhibition, antisera specific for idiotypic determinants of IgM-Gil-1970 were shown to be shared by IgM from 1970, 1972 and 1974 sera. The data support the notion that autoantibody-producing cell clones persist unaltered during the course of an untreated autoimmune disease, in this instance for 4 1/2 years.
对一名患有费尔蒂综合征的患者（吉尔）血清中获得的IgM抗IgG结构的持续性进行了研究。共等电聚焦实验表明，来自早期（1970年）和晚期（1973年）血清的完整IgM及其重链和轻链具有相同的等电点（pI）。1970年、1973年和1974年IgM-吉尔的轻链具有相同的氨基末端氨基酸序列，属于vκII轻链亚组。对从1970年、1972年和1974年获得的血清中分离出的IgM-吉尔的特异性研究表明，其与所有γ链亚类的IgG和IgG亚基具有相同的反应模式。最后，通过血凝抑制实验表明，1970年血清中IgM-吉尔独特型决定簇的特异性抗血清也存在于1970年、1972年和1974年血清的IgM中。这些数据支持了自身抗体产生细胞克隆在未经治疗的自身免疫性疾病过程中持续不变的观点，在这种情况下持续了4年半。
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