Johnston S L, Jorizzo J R, Liberman D F, Abraham G N
Immunology. 1979 Apr;36(4):707-10.
The co-isoelectric focusing characteristics of the antigen-IgG and residual IgG pools in the serum of patients with mixed IgM:IgG cryoglobulinemia have been determined. The antigen IgG purified from five IgM cryoglobulins and the residual IgG purified from serum depleted of cryoglobulin were radiolabelled and co-isoelectric focused in polyacrylamide gels in order to determine if the IgM anti-globulin reacted in order to determine if the IgM anti-globulin reacted with IgGs having unique physicochemical properties. Electrofocusing profiles of the IgG from four sera showed only minor differences when the antigen-IgG was compared to residual serum IgG. In one instance, the IEF profile of the antigen-IgG pool was markedly restricted and of a different charge dispersion than the residual serum IgG. This correlated with the limited antigenic specificity previously shown for the autologous IgM antiglobulin.
已测定了混合性IgM:IgG冷球蛋白血症患者血清中抗原-IgG和残余IgG池的共等电聚焦特性。从五种IgM冷球蛋白中纯化的抗原IgG和从去除冷球蛋白的血清中纯化的残余IgG用放射性标记，并在聚丙烯酰胺凝胶中共等电聚焦，以确定IgM抗球蛋白是否与具有独特物理化学性质的IgG发生反应。当将抗原-IgG与残余血清IgG进行比较时，来自四份血清的IgG的电聚焦图谱仅显示出微小差异。在一个实例中，抗原-IgG池的IEF图谱明显受限，且电荷分散与残余血清IgG不同。这与先前显示的自体IgM抗球蛋白有限的抗原特异性相关。
