Bechler B, Hunzinger E, Müller O, Cogoli A
Space Biology Group, ETH Zürich-Technopark, Switzerland.
Biol Cell. 1993;79(1):45-50. doi: 10.1016/0248-4900(93)90261-c.
The behaviour of two mammalian cell lines was investigated in Biorack during the 1st Spacelab international microgravity laboratory flight (IML-1) in the ESA facility Biorack. The parameters determined were cell proliferation, biosynthesis of specific cell products, consumption of glucose, glutamine and production of ammonia and lactate respectively. Murine Friend leukemia virus-transformed cells (Friend cells) were induced to differentiate and express hemoglobin (Hg) genes upon induction with dimethylsulfoxide (DMSO). No change was observed in all metabolic parameters including the production of Hg and the number of Hg-positive cells. Electron microscopy analysis showed no difference in morphology, mean cell volume and mitotic index between the different cell samples. Murine hybridoma cells revealed an increase (+ 30-40%) of cell proliferation rate in microgravity, whereas the metabolic parameters, production of monoclonal antibodies included, were lower in the 0 g than in the 1 g controls. The results clearly show that not all mammalian cells undergo dramatic changes in microgravity and that the effects reported on human T lymphocytes represent a unique case.
在欧洲航天局的生物舱设施中，于首次国际微重力实验室飞行（IML - 1）期间，对两种哺乳动物细胞系在生物舱中的行为进行了研究。所测定的参数分别为细胞增殖、特定细胞产物的生物合成、葡萄糖消耗、谷氨酰胺消耗以及氨和乳酸的产生。用二甲基亚砜（DMSO）诱导小鼠Friend白血病病毒转化细胞（Friend细胞）进行分化并表达血红蛋白（Hg）基因。在包括Hg产生和Hg阳性细胞数量在内的所有代谢参数方面均未观察到变化。电子显微镜分析表明，不同细胞样品在形态、平均细胞体积和有丝分裂指数上没有差异。小鼠杂交瘤细胞在微重力环境下细胞增殖速率增加（+30 - 40%），而包括单克隆抗体产生在内的代谢参数在0g条件下比1g对照时更低。结果清楚地表明，并非所有哺乳动物细胞在微重力环境下都会发生显著变化，并且所报道的对人类T淋巴细胞的影响代表了一种独特情况。
