Rao P H, Mathew S, Lauwers G, Rodriguez E, Kelsen D P, Chaganti R S
Laboratory of Cancer Genetics, Sloan-Kettering Institute, New York, NY 10021.
Diagn Mol Pathol. 1993 Dec;2(4):264-8.
Numerical changes affecting chromosomes 1, 2, 3, 4, 6, 7, 8, 10, 11, 12, 16, 17, 18, and the X and Y chromosomes have been analyzed using chromosome-specific centromeric alpha-satellite repeat DNA probes in a panel of biopsies of six gastric and three esophageal adenocarcinoma and one epidermoid carcinoma of esophagus obtained at surgery. For each case, with each probe, the number of hybridization signals were determined in 200 nuclei. Hybridization of each probe to phytohemagglutinin-stimulated normal peripheral blood lymphocytes served as controls. Monosomy was defined by loss of one signal in 15% or more cells and trisomy or tetrasomy was defined by the presence of 3 or 4 signals in 7% or more cells, respectively. The Y chromosome was lost in 6 of 8 cases and monosomy 10 was seen in 5 of 10 cases. Trisomy for chromosomes 17, 8, 7, 12, 11, and 1 was seen in 4 of 10, 4 of 10, 4 of 10, 2 of 10, 2 of 8, and 2 of 10 cases, respectively, and tetrasomy for chromosome 7 was seen in 1 of 10 cases. These data show that the Y chromosome and chromosomes 10, 8, 7, 17, and 12 are most frequently involved in nondisjunctional changes in these tumors. They also document the feasibility and utility of interphase cytogenetics of gastric adenocarcinomas.
利用染色体特异性着丝粒α-卫星重复DNA探针，对6例胃腺癌、3例食管腺癌和1例食管表皮样癌手术切除标本的活检组织进行分析，以研究影响1号、2号、3号、4号、6号、7号、8号、10号、11号、12号、16号、17号、18号染色体以及X和Y染色体的数目变化。对于每例标本，使用每种探针在200个细胞核中确定杂交信号的数量。每种探针与植物血凝素刺激的正常外周血淋巴细胞杂交作为对照。单倍体定义为15%或更多细胞中一个信号缺失，三体或四体分别定义为7%或更多细胞中出现3个或4个信号。8例中有6例Y染色体缺失，10例中有5例出现10号染色体单体。10例中有4例出现17号、8号、7号、12号、11号和1号染色体三体，10例中有4例、10例中有4例、10例中有4例、10例中有2例、8例中有2例和10例中有2例，10例中有1例出现7号染色体四体。这些数据表明，Y染色体以及10号、8号、7号、17号和12号染色体在这些肿瘤的非整倍体变化中最常受累。它们还证明了胃腺癌间期细胞遗传学的可行性和实用性。
