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通过二氯荧光素检测发现，结晶态的Ni3S2特别增强了CHO细胞核中氧化剂的形成。

Crystalline Ni3S2 specifically enhances the formation of oxidants in the nuclei of CHO cells as detected by dichlorofluorescein.

作者信息

Huang X, Klein C B, Costa M

机构信息

Nelson Institute of Environmental Medicine, New York University Medical Center, NY 10016.

出版信息

Carcinogenesis. 1994 Mar;15(3):545-8. doi: 10.1093/carcin/15.3.545.

DOI:10.1093/carcin/15.3.545
PMID:8118941
Abstract

Dichlorofluorescein (DCF) was used as a fluorescent probe to detect oxidants formed in cultured CHO cells during nickel treatment. Crystalline Ni3S2 specifically enhanced the formation of oxidants in the nuclei of these living cells, but Ni3S2 particles did not enhance DCF fluorescence as much when added in vitro to isolated nuclei. Our results add to the emerging concept that oxidants mediated by nickel compounds may play an important role in nickel-induced genotoxicity.

摘要

二氯荧光素（DCF）被用作荧光探针，以检测镍处理过程中培养的CHO细胞中形成的氧化剂。结晶态的Ni3S2特异性增强了这些活细胞细胞核中氧化剂的形成，但当在体外添加到分离的细胞核中时，Ni3S2颗粒对DCF荧光的增强作用没有那么大。我们的结果进一步支持了这一新兴概念，即镍化合物介导的氧化剂可能在镍诱导的遗传毒性中起重要作用。

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