Bromberg J S, Schachner M
Invest Ophthalmol Vis Sci. 1978 Sep;17(9):920-4.
Several antigens expressed in the nervous system were localized in tissue sections of developing and adult mouse retina by indirect immunofluorescence. Two antigens expressed in oligodendrocytes, basic protein of myelin, and NS-1 are not detectable. Antisera against nervous system-3, -4, and -7 antigens (NS-3, NS-4, and NS-7) give a uniformly intense reaction on all retinal cell structures. Large, external, transformation-sensitive (LETS) protein is present on blood vessels. Glial fibrillary acidic (GFA) protein is absent at birth but is found after day 4 in cells of the ganglion cell and nerve fiber layers. GFA protein--positive cells extend in parallel from inner to outer limiting membranes only at the ora serrata and around the optic nerve. No other glial elements contain GFA antigen, suggesting two distinct populations of astrocytes. Neurofilament (NF) protein--positive cells are present in the adult retina in outer plexiform and ganglion cell layers. By day 4 the ganglion cell layer and the ependymal zone have become NF-positive.
通过间接免疫荧光法，在发育中和成年小鼠视网膜的组织切片中定位了几种在神经系统中表达的抗原。在少突胶质细胞中表达的两种抗原，即髓磷脂碱性蛋白和NS-1，无法检测到。针对神经系统-3、-4和-7抗原（NS-3、NS-4和NS-7）的抗血清在所有视网膜细胞结构上均产生均匀强烈的反应。大的、外部的、转化敏感（LETS）蛋白存在于血管上。胶质纤维酸性（GFA）蛋白在出生时不存在，但在出生后第4天在神经节细胞层和神经纤维层的细胞中发现。GFA蛋白阳性细胞仅在锯齿缘和视神经周围从内界膜到外界膜平行延伸。没有其他神经胶质成分含有GFA抗原，这表明存在两种不同的星形胶质细胞群体。神经丝（NF）蛋白阳性细胞存在于成年视网膜的外网状层和神经节细胞层。到第4天时，神经节细胞层和室管膜区已变为NF阳性。
