Eliopoulos G D, Vaiopoulos G, Eliopoulos A G, Kyriakou D, Katrinakis G, Alexandrakis M
Department of Haematology, University of Crete School of Medicine, University Hospital of Heraklion, Greece.
Haematologia (Budap). 1993;25(3):215-22.
Natural killer cell activity (NKa) and mitogen-induced cellular cytotoxicity (MICC) of peripheral blood mononuclear cells (PBMC) were studied in five patients with chronic LGL-proliferative disease (LGL-PD) of the CD3+, CD8+, CD57+ phenotype. Both assays were performed under the same experimental conditions except that cultures for MICC contained phytohemagglutinin (PHA) at varying concentrations. Cytotoxicity was assessed against K562 cell targets using the 18 hours 51-chromium release assay. We found that LGL-PD lymphocytes of the aforementioned phenotype express low NKa but high MICC. Furthermore, supernatants derived from patients' PMBC cultures stimulated with PHA, displayed cytolytic properties comparable to those of normal lymphocytes. The findings indicate that MICC may be mediated, at least partially, by humoral cytolytic molecules. We concluded that LGL-PD lymphocytes are unable to express natural cytotoxicity but they have not lost the cytolytic machinery necessary for the destruction of sensitive target cells.
对5例具有CD3+、CD8+、CD57+表型的慢性大颗粒淋巴细胞增殖性疾病（LGL-PD）患者的外周血单个核细胞（PBMC）的自然杀伤细胞活性（NKa）和丝裂原诱导的细胞毒性（MICC）进行了研究。除了MICC培养物含有不同浓度的植物血凝素（PHA）外，两种检测均在相同的实验条件下进行。使用18小时51铬释放试验评估对K562细胞靶标的细胞毒性。我们发现，上述表型的LGL-PD淋巴细胞表达低NKa但高MICC。此外，来自患者PHA刺激的PBMC培养物的上清液表现出与正常淋巴细胞相当的溶细胞特性。这些发现表明，MICC可能至少部分地由体液溶细胞分子介导。我们得出结论，LGL-PD淋巴细胞无法表达自然细胞毒性，但它们并未丧失破坏敏感靶细胞所需的溶细胞机制。
