Eliopoulos G, Constantopoulou M, Coulocheri S, Vaiopoulos G, Eliopoulos A, Yataganas X
Department of Hematology, University of Crete School of Medicine, University Hospital of Heraklion, Greece.
Anticancer Res. 1991 Mar-Apr;11(2):685-9.
Peripheral blood mitogen - induced cellular cytotoxicity (MICC) and natural killer- cell cytotoxicity (NKCC) were assessed in 25 patients with myelodysplastic syndromes (MDS). Both MICC and NKCC were examined under the same experimental conditions using the 18 hr chromium release assay, except that cultures for MICC were stimulated in vitro by the addition of phytohemagglutinin (PHA). Patients' MICC was found significantly reduced, in relation to controls (p less than 0.001), but significantly higher than patients' NKCC (p less than 0.001). Furthermore, patients CD3+ cells and CD4+ cells, as well as the CD4+/CD8+ ratio, were significantly decreased (p less than 0.01, p less than 0.001 and p less than 0.001, respectively), while CD8+ cells and CD16+ cells were within normal limits. No relationship was noted between patients' MICC and total lymphocyte count or any lymphocyte subpopulation. In eleven patients who were subsequently subjected to a-interferon (a-IFN) administration, MICC values were found within normal range one month after the cessation of alpha-IFN, while NKCC values were significantly increased (p less than 0.01), but they still remained below the lower limit of the control (p less than 0.001). Percentages of CD3+, CD4+ and CD8+ cells, as well as the CD4+/CD8+ ratio, did not change after alpha-IFN, but the absolute numbers of CD3+ cells and CD8+ cells were significantly reduced. A statistically significant rise was noted in CD16+ cells. Post- IFN rises in MICC did not correlate with lymphocyte subpopulations. The findings indicate that MDS patients display very low MICC, which can be restored by alpha-IFN administration. The cause of this disturbance and the mechanism of its restoration by alpha-IFN remain unclear.
对25例骨髓增生异常综合征（MDS）患者的外周血有丝分裂原诱导的细胞毒性（MICC）和自然杀伤细胞毒性（NKCC）进行了评估。除了用于MICC的培养物在体外通过添加植物血凝素（PHA）进行刺激外，在相同的实验条件下使用18小时铬释放试验对MICC和NKCC进行检测。发现患者的MICC相对于对照组显著降低（p<0.001），但显著高于患者的NKCC（p<0.001）。此外，患者的CD3+细胞和CD4+细胞以及CD4+/CD8+比值显著降低（分别为p<0.01、p<0.001和p<0.001），而CD8+细胞和CD16+细胞在正常范围内。未发现患者的MICC与总淋巴细胞计数或任何淋巴细胞亚群之间存在关联。在随后接受α-干扰素（α-IFN）治疗的11例患者中，发现停止使用α-IFN一个月后MICC值在正常范围内，而NKCC值显著升高（p<0.01），但仍低于对照组的下限（p<0.001）。α-IFN治疗后，CD3+、CD4+和CD8+细胞的百分比以及CD4+/CD8+比值没有变化，但CD3+细胞和CD8+细胞的绝对数量显著减少。CD16+细胞有统计学意义的升高。IFN治疗后MICC的升高与淋巴细胞亚群无关。这些发现表明，MDS患者表现出非常低的MICC，α-IFN治疗可使其恢复。这种紊乱的原因及其通过α-IFN恢复的机制尚不清楚。
