Pang C S, Brown G M, Tang P L, Pang S F
Clarke Institute of Psychiatry, Department of Neuroendocrinology, Toronto, Ont., Canada.
Neurosci Lett. 1993 Nov 12;162(1-2):17-20. doi: 10.1016/0304-3940(93)90549-z.
[125I]Iodomelatonin binding was first demonstrated in the chicken lung membrane preparations. The binding was saturable, reversible, rapid, and of high affinity. The sites of binding distributed widely in different subcellular fractions except the cytosol fraction. Scatchard plots are linear indicating a dissociation constant (Kd) of 8.11 +/- 0.73 pmol/l and a maximum number of binding sites of 1.29 +/- 0.16 fmol/mg protein (n = 9). The Kd estimated from the kinetic study was 11.2 pmol/l. Guanosine 5'-O-(3-thiotriphosphate) significantly increased the Kd values while the density of binding sites was not affected indicating that the binding sites may be linked to a guanine nucleotide binding protein.
[125I]碘褪黑素结合首先在鸡肺膜制剂中得到证实。这种结合具有饱和性、可逆性、快速性和高亲和力。结合位点广泛分布于除细胞质部分以外的不同亚细胞组分中。Scatchard图呈线性，表明解离常数（Kd）为8.11±0.73 pmol/l，最大结合位点数为1.29±0.16 fmol/mg蛋白质（n = 9）。动力学研究估计的Kd为11.2 pmol/l。鸟苷5'-O-(3-硫代三磷酸)显著增加了Kd值，而结合位点密度未受影响，这表明结合位点可能与鸟嘌呤核苷酸结合蛋白相关。
