Kawabata T, Yap S C, Packer L
Department of Molecular and Cell Biology, University of California, Berkeley 94720.
Biochem Biophys Res Commun. 1994 Feb 28;199(1):361-7. doi: 10.1006/bbrc.1994.1237.
Because iron is involved in catalysis of many biological oxidations, it is important to investigate new and novel antioxidants in terms of their effect on iron-catalyzed oxidations. We investigated the effect of dihydrolipoic acid (6,8-dimercaptooctanoic acid (DHLA)), its homologues (4,6-dimercaptohexanoic acid (bisnorDHLA) and 2,4-dimercaptobutanoic acid (tetranorDHLA)) and methyl 6,8-dimercaptooctanoate (methylDHLA) on Fe(II)-citrate-catalyzed lipid peroxide-dependent lipid peroxidation in lipid-dispersed and liposome systems. In the lipid-dispersed system, tetranorDHLA inhibited conjugated diene formation induced by Fe(II)-citrate. In the presence of tetranorDHLA, oxygen was consumed more rapidly in the reaction mixture than in the presence of the other compounds, but the oxidation rate of Fe(II)-citrate in the reaction mixture was slower than in the presence of the other compounds. This suggests that tetranorDHLA inhibited lipid peroxidation by the oxidation of tetranorDHLA itself at the site where the lipid was oxidized.
由于铁参与多种生物氧化的催化过程，因此从其对铁催化氧化的影响方面研究新型抗氧化剂具有重要意义。我们研究了二氢硫辛酸（6,8 - 二巯基辛酸（DHLA））、其同系物（4,6 - 二巯基己酸（双去甲DHLA）和2,4 - 二巯基丁酸（四去甲DHLA））以及6,8 - 二巯基辛酸甲酯（甲基DHLA）对脂质分散体系和脂质体体系中柠檬酸铁（II）催化的脂质过氧化物依赖性脂质过氧化反应的影响。在脂质分散体系中，四去甲DHLA抑制了柠檬酸铁（II）诱导的共轭二烯形成。在四去甲DHLA存在的情况下，反应混合物中氧气的消耗速度比其他化合物存在时更快，但反应混合物中柠檬酸铁（II）的氧化速率比其他化合物存在时更慢。这表明四去甲DHLA在脂质被氧化的部位通过自身氧化抑制了脂质过氧化。
