Verhoeven A J, Jansen H
Department of Biochemistry, Erasmus University Rotterdam, The Netherlands.
Biochim Biophys Acta. 1994 Feb 10;1211(1):121-4. doi: 10.1016/0005-2760(94)90146-5.
Rat and human steroidogenic organs contain an enzyme activity that is indistinguishable from hepatic lipase present in liver. Using primers that recognize exons 5 and 8 of the rat and human HL gene, a 596-bp product was found by RT-PCR in rat liver, adrenal, ovaries and testes, but not in heart and kidney. A similar product was also observed with human hyperplastic adrenocortical tissue. Identity of this product with part of the HL cDNA was confirmed by restriction mapping and internal re-amplification. Our results indicate that the HL gene is transcribed in steroidogenic tissues that also contain HL protein.
大鼠和人类的类固醇生成器官含有一种酶活性，该活性与肝脏中存在的肝脂肪酶无法区分。使用识别大鼠和人类HL基因外显子5和8的引物，通过逆转录聚合酶链反应（RT-PCR）在大鼠肝脏、肾上腺、卵巢和睾丸中发现了一个596碱基对的产物，但在心脏和肾脏中未发现。在人类增生性肾上腺皮质组织中也观察到了类似的产物。通过限制性酶切图谱分析和内部重新扩增，证实了该产物与HL cDNA的一部分具有同一性。我们的结果表明，HL基因在同时含有HL蛋白的类固醇生成组织中被转录。
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