Kelly J W, He L, Stewart J T
Medicinal Chemistry Department, College of Pharmacy, University of Georgia, Athens 30602-2352.
J Pharm Biomed Anal. 1993 Nov-Dec;11(11-12):1141-4. doi: 10.1016/0731-7085(93)80095-i.
R(-) and S(+) praziquantel (PZQ) in human serum were resolved and quantified using a stereospecific LC method. Each enantiomer and the internal standard 2-methylamino-5-chlorobenzophenone were isolated from serum using a solid-phase extraction procedure on a cyanopropyl column. Recoveries of 98.8, 93.3 and 35.4% were obtained for R(-) PZQ, S(+) PZQ, and the internal standard, respectively. A cellulose-based chiral analytical column (Chiralcel OD) was used with a mobile phase consisting of hexane-2-propanol (70:30, v/v). Linear calibration curves were obtained in the concentration range 50-1000 ng ml-1 for each enantiomer in serum. The detection limit for each enantiomer in serum using UV detection at 212 nm was 5 ng ml-1 (S/N = 3). The limit of quantitation of each enantiomer was 25 ng ml-1.
采用立体特异性液相色谱法拆分并定量测定人血清中的R(-)和S(+)吡喹酮（PZQ）。使用氰丙基柱上的固相萃取程序从血清中分离出每种对映体和内标2-甲基氨基-5-氯二苯甲酮。R(-) PZQ、S(+) PZQ和内标的回收率分别为98.8%、93.3%和35.4%。使用基于纤维素的手性分析柱（Chiralcel OD），流动相由己烷-2-丙醇（70:30，v/v）组成。血清中每种对映体在50-1000 ng ml-1浓度范围内获得线性校准曲线。在212 nm处使用紫外检测时，血清中每种对映体的检测限为5 ng ml-1（信噪比=3）。每种对映体的定量限为25 ng ml-1。
