Fieger H, Wainer I W
McGill University, Department of Oncology, Montreal General Hospital, Quebec, Canada.
J Pharm Biomed Anal. 1993 Nov-Dec;11(11-12):1173-9. doi: 10.1016/0731-7085(93)80101-6.
A high-performance liquid chromatographic assay has been developed for the quantification of the enantiomers of mexiletine and its four major metabolites, in plasma and in urine. Mexiletine and all metabolites were determined, after derivatization of mexiletine and its hydroxymetabolites, p-hydroxymexiletine and hydroxymethylmexiletine, using a Chiralpak AD chiral stationary phase, based on a carbamoyl derivative of amylose. o-phthalaldehyde was chosen as derivatization reagent to increase the sensitivity of detection, to achieve separation of all compounds in one chromatographic system, and to avoid interferences.
已开发出一种高效液相色谱分析法，用于定量测定血浆和尿液中美西律及其四种主要代谢物的对映体。在美西律及其羟基代谢物对羟基美西律和羟甲基美西律衍生化后，使用基于直链淀粉氨基甲酰衍生物的Chiralpak AD手性固定相测定美西律和所有代谢物。选择邻苯二甲醛作为衍生化试剂，以提高检测灵敏度，在一个色谱系统中实现所有化合物的分离，并避免干扰。
