妊娠区带蛋白的亲和层析纯化
Affinity chromatographic purification of the pregnancy zone protein.
作者信息
Folkersen J, Teisner B, Ahrons S, Svehag S E
出版信息
J Immunol Methods. 1978;23(1-2):117-25. doi: 10.1016/0022-1759(78)90115-1.
PMID:81241
Abstract
An immunospecific affinity chromatographic method for purification of human pregnancy zone protein (PZP) directly from serum is described. Highly purified goat-anti-human PZP-immunoglobulin was applied as a ligand. Recovery of PZP varied from 56--75%. The impurities constituted maximally 5--10% of the total protein in the eluate. The purification factor was approximately 100.
摘要
本文描述了一种直接从血清中纯化人妊娠区带蛋白（PZP）的免疫特异性亲和色谱法。使用高度纯化的山羊抗人PZP免疫球蛋白作为配体。PZP的回收率在56%至75%之间。洗脱液中杂质最多占总蛋白的5%至10%。纯化因子约为100。
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