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外周血淋巴细胞中一种葡萄糖结合凝集素的研究。

Studies on a glucose-binding lectin from peripheral blood lymphocytes.

作者信息

Kayestha R, Berry A, Hajela K

机构信息

School of Life Sciences, Devi Ahilya Vishwavidyalay, Vigyan Bhawan, Indore, India.

出版信息

Immunol Lett. 1993 Nov;38(3):201-5. doi: 10.1016/0165-2478(93)90007-o.

DOI:10.1016/0165-2478(93)90007-o
PMID:8125528
Abstract

Lectin-carbohydrate interactions have been found to be important in many of the steps of lymphocyte recirculation and inflammatory responses. A D-glucose-specific lectin was isolated from goat peripheral blood lymphocytes by affinity chromatography on N-acetyl-D-glucosamine agarose and gave a single band corresponding to 112 kDa in SDS-PAGE, irrespective of treatment with 2-mercaptoethanol. The lymphocyte lectin agglutinated rabbit and human ABO erythrocytes, the hemagglutinating activity being Ca2+ dependent. It appears to be a member of type C animal lectins.

摘要

凝集素与碳水化合物的相互作用在淋巴细胞再循环和炎症反应的许多步骤中都很重要。通过在N-乙酰-D-葡萄糖胺琼脂糖上进行亲和层析，从山羊外周血淋巴细胞中分离出一种D-葡萄糖特异性凝集素，在SDS-PAGE中呈现出一条对应于112 kDa的单带，无论是否用2-巯基乙醇处理。该淋巴细胞凝集素能凝集兔和人的ABO红细胞，其血凝活性依赖于Ca2+。它似乎是C型动物凝集素的一员。

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