Antunes-Rodrigues J, Picanco-Diniz D W, Favaretto A L, Gutkowska J, McCann S M
Department of Physiology, School of Medicine, University of Saõ Paulo, Ribeiraõ Preto, Brazil.
Neuroendocrinology. 1993 Dec;58(6):696-700. doi: 10.1159/000126611.
The brain atrial natriuretic peptide (ANP) neuronal system appears to be involved in the increase in plasma ANP which follows blood volume expansion in the rat. To determine if this neuronal system is essential to the natriuresis and increase in plasma ANP which follow volume expansion, highly specific antiserum against ANP (ANP-AB) and/or normal rabbit serum as a control was microinjected into the third cerebral ventricle (3V) of conscious rats, and the effect on the natriuresis and increase in plasma ANP induced by intravenous injection of 2 ml/100 g body weight of 0.3 M NaCl was examined. Although there was no effect of ANP-AB on initial levels of plasma ANP or natriuresis 3 h after 3V injection, the natriuresis in response to blood volume expansion was significantly inhibited. The increase in plasma ANP which followed volume expansion was also significantly reduced at 5 min but recovered at 15 min. The results indicate that the brain ANP neuronal system plays an essential role in the mediation of volume expansion-induced increase in plasma ANP and natriuresis. The failure to block these responses completely may be due to the use of an inadequate dose of antiserum or other brain mechanisms may be able to mediate these responses.
脑利钠肽（ANP）神经元系统似乎参与了大鼠血容量扩张后血浆ANP的增加。为了确定该神经元系统对于容量扩张后利钠作用及血浆ANP增加是否至关重要，将高特异性抗ANP血清（ANP-AB）和/或作为对照的正常兔血清微量注射到清醒大鼠的第三脑室（3V），并检测其对静脉注射2 ml/100 g体重的0.3 M NaCl诱导的利钠作用及血浆ANP增加的影响。尽管3V注射3小时后ANP-AB对血浆ANP初始水平或利钠作用无影响，但对血容量扩张的利钠反应受到显著抑制。容量扩张后血浆ANP的增加在5分钟时也显著降低，但在15分钟时恢复。结果表明，脑ANP神经元系统在介导容量扩张诱导的血浆ANP增加和利钠作用中起重要作用。未能完全阻断这些反应可能是由于使用的抗血清剂量不足，或者可能存在其他脑机制介导这些反应。
