人类促甲状腺激素释放激素（TRH）受体基因定位于8号染色体。

Assignment of human thyrotropin-releasing hormone (TRH) receptor gene to chromosome 8.

作者信息

Yamada M, Monden T, Konaka S, Mori M

机构信息

First Department of Internal Medicine, Gunma University School of Medicine, Japan.

出版信息

Somat Cell Mol Genet. 1993 Nov;19(6):577-80. doi: 10.1007/BF01233384.

DOI:10.1007/BF01233384

PMID:8128317

Abstract

The human gene encoding thyrotropin-releasing hormone receptor was assigned to chromosome 8, using human-Chinese hamster ovary somatic cell hybrids, analyzed by Southern hybridizations. Hybridization was carried out with a 32P-labeled fragment of the human TRH-R genomic DNA. Hybridization of this probe to a human specific 10.5-kb DNA fragment of EcoRI-digested WBC DNA was used to localize the human TRH-R gene. No hybridization, by contrast, was seen with this probe and hamster DNA after EcoRI treatment. Results from 18 somatic cell hybrids corroborated unequivocally that the human TRH-R gene can be assigned to human chromosome 8.

摘要

利用人-中国仓鼠卵巢体细胞杂种，通过Southern杂交分析，将编码促甲状腺激素释放激素受体的人类基因定位于8号染色体。用32P标记的人类促甲状腺激素释放激素受体（TRH-R）基因组DNA片段进行杂交。该探针与经EcoRI消化的白细胞DNA的一条10.5kb人类特异性DNA片段杂交，以此来定位人类TRH-R基因。相比之下，经EcoRI处理后的该探针与仓鼠DNA未见杂交。18个体细胞杂种的结果明确证实，人类TRH-R基因可定位于人类8号染色体。