Nickerson S C, Owens W E, Rejman J J, Oliver S P
Mastitis Research Laboratory, Louisiana Agricultural Experiment Station, Louisiana State University Agricultural Center, Homer 71040.
Zentralbl Veterinarmed B. 1993 Dec;40(9-10):621-33. doi: 10.1111/j.1439-0450.1993.tb00185.x.
Eight Jersey cows free of intramammary infection (IMI) with major mastitis pathogens were intramammarily infused with interleukin (IL)-1, IL-2, or phosphate-buffered saline (PBS) after the last milking prior to drying off. Rectal temperatures were monitored hourly through 6 hours after infusion and at 24-hour intervals thereafter. Quarter secretions were collected 7 days prior to drying off, at drying off, and on days 1, 2, 3, 4, 7, and 14 of the nonlactating period. Cows were slaughtered and mammary tissues were collected at 7 (3 cows) and 14 (5 cows) days after treatment. The infusion of 10 micrograms of IL-1 produced an elevation in rectal temperature that was significantly higher than that of cows receiving IL-2 or PBS at 4, 5, and 6 hours after treatment, whereas cows receiving 1.0 mg of IL-2 exhibited no change in rectal temperature. Somatic cell counts (SCC) in mammary glands infused with IL-1 or IL-2 remained significantly elevated over PBS-infused control glands through day 4 after treatment. Neutrophils predominated in secretions of IL-1-treated glands, and macrophages and lymphocytes predominated in IL-2-treated glands. The mammary involution process was accelerated by IL-2, and tissues exhibited reductions in alveolar epithelial and luminal areas and increases in connective tissue stroma and degree of leukocytosis compared with IL-1 and control glands. Concentrations of plasma cells producing IgG1, IgG2, IgA, and IgM also were greatest following IL-2 infusion. Results suggest that IL-2 is superior to IL-1 in stimulating an increase in mammary mononuclear cell populations and in accelerating involution during the early nonlactating period.
八头无乳房内感染（IMI）且无主要乳腺炎病原体的泽西奶牛，在干奶前最后一次挤奶后，通过乳腺内注射白细胞介素（IL）-1、IL-2或磷酸盐缓冲盐水（PBS）。注射后每小时监测直肠温度，持续6小时，之后每隔24小时监测一次。在干奶前7天、干奶时以及非泌乳期的第1、2、3、4、7和14天收集乳腺分泌物。在治疗后7天（3头奶牛）和14天（5头奶牛）宰杀奶牛并收集乳腺组织。注射10微克IL-1后，直肠温度升高，在治疗后4、5和6小时显著高于接受IL-2或PBS的奶牛，而接受1.0毫克IL-2的奶牛直肠温度无变化。与注射PBS的对照腺体相比，注射IL-1或IL-2的乳腺中的体细胞计数（SCC）在治疗后第4天仍显著升高。IL-1处理腺体的分泌物中以中性粒细胞为主，IL-2处理腺体的分泌物中以巨噬细胞和淋巴细胞为主。IL-2加速了乳腺退化过程，与IL-1和对照腺体相比，组织的肺泡上皮和管腔面积减小，结缔组织基质增加，白细胞增多程度增加。注射IL-2后，产生IgG1、IgG2、IgA和IgM的浆细胞浓度也最高。结果表明，在刺激乳腺单核细胞群体增加以及在非泌乳早期加速退化方面，IL-2优于IL-1。
