Quiroga G H, Nickerson S C, Adkinson R W
Louisiana Agricultural Experiment Station, Louisiana State University Agricultural Center, Homer 71040.
J Dairy Sci. 1993 Oct;76(10):2913-24. doi: 10.3168/jds.S0022-0302(93)77631-6.
The histologic and cytologic responses of heifer mammary glands infected with Staphylococcus aureus were studied after infusion of interleukin-2 or interferon-gamma. Two groups of 4 heifers each, which were infected experimentally with S. aureus in all 4 mammary quarters, were infused in diagonal quarters with 7.5 x 10(5) units of interleukin-2 or 10(5) units of interferon-gamma; remaining quarters received PBS. Heifers in both trials were slaughtered 14 d after cytokine infusion, and mammary tissues were collected for histological examination. Uninfected quarters from 2 additional heifers were left untreated to compare infected with uninfected tissues for both trials. Morphologic evaluation and leukocyte infiltration scores were performed on tissue sections stained with hematoxylin and eosin, and plasma cells were quantified on sections stained with immunoperoxidase. Infected quarters had lower percentages of alveolar epithelial and luminal areas and higher percentages of stromal area than did uninfected quarters in the interleukin-2 trial, but no differences were observed between infected quarters that had been treated with PBS or interleukin-2. Likewise, interferon-gamma treatment had no effect on mammary parenchymal components in the infected quarters. Interleukin-2 treatment significantly elevated leukocytosis into the mammary gland parenchyma compared with infected quarters treated with PBS and uninfected quarters. Among the leukocyte types evaluated, eosinophilic infiltration was elevated in interleukin-2 quarters over that of PBS controls. In both trials, concentrations of plasma cells bearing Ig were elevated in infected versus uninfected quarters. Plasma cell concentrations also were higher in cytokine than PBS controls, especially in interleukin-2 quarters. Results suggested that neither cytokine influenced the histology of infected mammary tissues, but both interleukin-2 and interferon-gamma increased, although insignificantly, the prevalence of all isotypes of plasma cells bearing Ig, suggesting enhancement of the local immune response to IMI.
在注入白细胞介素-2或γ-干扰素后，对感染金黄色葡萄球菌的小母牛乳腺的组织学和细胞学反应进行了研究。将两组各4头小母牛在所有4个乳腺象限中进行金黄色葡萄球菌的实验性感染，在对角象限中注入7.5×10⁵单位的白细胞介素-2或10⁵单位的γ-干扰素；其余象限注入磷酸盐缓冲盐水（PBS）。在细胞因子注入后14天宰杀两个试验中的小母牛，并收集乳腺组织进行组织学检查。另外选取2头小母牛未感染的象限不进行处理，以便在两个试验中比较感染组织与未感染组织。对苏木精和伊红染色的组织切片进行形态学评估和白细胞浸润评分，并对免疫过氧化物酶染色的切片上的浆细胞进行定量。在白细胞介素-2试验中，感染象限的肺泡上皮和管腔面积百分比低于未感染象限，而基质面积百分比高于未感染象限，但在注入PBS或白细胞介素-2的感染象限之间未观察到差异。同样，γ-干扰素处理对感染象限的乳腺实质成分没有影响。与注入PBS的感染象限和未感染象限相比，白细胞介素-2处理显著提高了乳腺实质中的白细胞增多。在所评估的白细胞类型中，白细胞介素-2处理的象限中嗜酸性粒细胞浸润高于PBS对照。在两个试验中，感染象限中携带免疫球蛋白（Ig）的浆细胞浓度均高于未感染象限。细胞因子处理组的浆细胞浓度也高于PBS对照组，尤其是在白细胞介素-2处理的象限。结果表明，两种细胞因子均未影响感染乳腺组织的组织学，但白细胞介素-2和γ-干扰素均增加了（尽管不显著）携带Ig的所有浆细胞同种型的患病率，表明对传染性乳房炎的局部免疫反应增强。
