抗着丝粒自身抗体。使用重组融合蛋白CENP - B作为抗原的酶联免疫吸附测定法的评估。
Anticentromere autoantibodies. Evaluation of an ELISA using recombinant fusion protein CENP-B as antigen.
作者信息
Vázquez-Abad D, Wallace S, Senécal J L, Joyal F, Roussin A, Earnshaw W C, Rothfield N
机构信息
University of Connecticut School of Medicine, Farmington.
出版信息
Arthritis Rheum. 1994 Feb;37(2):248-52. doi: 10.1002/art.1780370214.
OBJECTIVE
To evaluate an enzyme-linked immunosorbent assay (ELISA) for anticentromere autoantibodies (ACA).
METHODS
Sera from 611 patients with scleroderma, CREST (calcinosis, Raynaud's phenomenon, esophageal dysmotility, sclerodactyly, telangiectasias), Raynaud's disease, and connective tissue disease control patients were studied by ELISA using the fusion protein CENP-B, by immunofluorescence on dividing HEp-2 cells, and by immunoblotting on chromosomes and CENP-B.
RESULTS
Compared with immunofluorescence, the CENP-B ELISA sensitivity was 94% and the specificity was 93%. In 19.7% of the cases, there was a probability of a false-positive result and in 1.9%, a probability of a false-negative result, yielding positive and negative predictive values of 0.80 and 0.98, respectively.
CONCLUSION
The CENP-B ELISA is a sensitive and specific assay for ACA.
目的
评估一种用于检测抗着丝粒自身抗体（ACA）的酶联免疫吸附测定（ELISA）。
方法
采用融合蛋白CENP - B，通过ELISA对611例硬皮病、CREST综合征（钙质沉着、雷诺现象、食管运动障碍、指端硬化、毛细血管扩张）、雷诺病患者以及结缔组织病对照患者的血清进行检测；通过对分裂期HEp - 2细胞进行免疫荧光检测；通过对染色体和CENP - B进行免疫印迹检测。
结果
与免疫荧光法相比，CENP - B ELISA的敏感性为94%，特异性为93%。在19.7%的病例中，存在假阳性结果概率，在1.9%的病例中，存在假阴性结果概率，阳性预测值和阴性预测值分别为0.80和0.98。
结论
CENP - B ELISA是一种检测ACA的敏感且特异的检测方法。
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