Kremer L, Alvaro-Gracia J M, Ossorio C, Avila J
Centro de Biología Molecular (CSIC-UAM), Universidad Autónoma, Madrid, Spain.
Clin Exp Immunol. 1988 Jun;72(3):465-9.
Anticentromere antibodies (ACA) present in a high percentage of patients with complete or incomplete CREST scleroderma, and which are presently used in the diagnosis of this disease, also appear in some primary Raynaud's phenomenon patients. Three principal centromeric antigens, CENP-A, CENP-B and CENP-C, have been described as reacting with the sera of these individuals. We attempt to determine whether or not a correlation between the presence of ACA and serum reactivity against one or more of these peptides could be established, and have observed that CENP-A, but not CENP-B or CENP-C, is specifically recognized by all patients sera tested. The fact that this reactivity is clearly detectable at very high serum dilutions, thus eliminating other non-specific interference, suggests that anti-CENP-A activity might be useful in the diagnosis of patients with CREST-associated Raynaud's phenomenon.
抗着丝点抗体（ACA）在大部分完全型或不完全型CREST硬皮病患者中存在，目前用于该疾病的诊断，在一些原发性雷诺现象患者中也会出现。三种主要的着丝粒抗原，即CENP - A、CENP - B和CENP - C，已被描述为与这些个体的血清发生反应。我们试图确定ACA的存在与针对这些肽段中一种或多种的血清反应性之间是否存在相关性，并观察到所有检测的患者血清均能特异性识别CENP - A，而不是CENP - B或CENP - C。这种反应性在非常高的血清稀释度下仍能清晰检测到，从而消除了其他非特异性干扰，这一事实表明抗CENP - A活性可能有助于诊断与CREST相关的雷诺现象患者。
