在正相和反相纤维素手性固定相上通过高效液相色谱法直接对贝凡洛尔进行对映体选择性分离。

Direct enantioselective separation of bevantolol by high-performance liquid chromatography on normal and reverse cellulose chiral stationary phases.

作者信息

Aboul-Enein H Y, Serignese V

机构信息

Biological and Medical Research Department, King Faisal Specialist Hospital and Research Centre, Riyadh, Kingdom of Saudi Arabia.

出版信息

Biomed Chromatogr. 1994 Jan-Feb;8(1):22-5. doi: 10.1002/bmc.1130080106.

DOI:10.1002/bmc.1130080106

PMID:8130628

Abstract

A direct, isocratic and simple chromatographic method is described for the enantiomeric separation of bevantolol (BEV) using normal and reverse cellulose tris (3,5-dimethylphenylcarbamate) chiral stationary phases (CSPs) known as Chiralcel OD and Chiralcel OD-R, respectively. The effect of various alcohols present in the mobile phase on the separation factor (a) and resolution factor (Rs) has been studied. The method has been used to determine and identify the BEV enantiomers in human urine after oral administration of racemic BEV. The chiral recognition mechanism(s) between the analyte and these chiral stationary phases is proposed.

摘要

描述了一种直接、等度且简单的色谱方法，用于使用分别称为Chiralcel OD和Chiralcel OD-R的常规和反相纤维素三（3,5-二甲基苯基氨基甲酸酯）手性固定相（CSP）对贝凡洛尔（BEV）进行对映体分离。研究了流动相中存在的各种醇对分离因子（α）和分离度因子（Rs）的影响。该方法已用于在口服消旋BEV后测定和鉴定人尿中的BEV对映体。提出了分析物与这些手性固定相之间的手性识别机制。