Komatsu H, Takahata T, Tanaka M, Ishimitsu S, Okada S
Division of Drugs, National Institute of Health Science, Osaka, Japan.
Biol Pharm Bull. 1993 Dec;16(12):1189-93. doi: 10.1248/bpb.16.1189.
The molecular-weight distribution of commercially available low-molecular-weight heparins (LMWHs) was estimated by high-performance gel permeation chromatography (HPGPC) using a LMWH molecular-weight calibrant, supplied by the National Institute for Biological Standards and Control for the calibration of columns. This calibrant is a mixture of many polysaccharides with a disaccharide unit prepared by a random depolymerization of unfractionated heparin, and the ends of the fragments have a UV absorption. The ratio of the refractive index to the UV absorption is a relative measure of molecular weight. Various molecular-weight parameters such as weight-, number- or z-average molecular-weights, polydispersity and relative distributions of high, middle and low molecular-weight components were estimated, indicating that LMWHs have a wide variation in molecular-weight distribution. This variation was ascribed to the difference in preparation methods from unfractionated heparins.
采用由国家生物标准与控制研究所提供的低分子量肝素（LMWH）分子量校准物，通过高效凝胶渗透色谱法（HPGPC）对市售低分子量肝素的分子量分布进行了估算，该校准物用于柱校准。该校准物是通过对未分级肝素进行随机解聚制备的许多具有二糖单元的多糖的混合物，片段末端具有紫外吸收。折射率与紫外吸收的比值是分子量的相对度量。估算了各种分子量参数，如重量平均分子量、数均分子量或z均分子量、多分散性以及高分子量、中分子量和低分子量组分的相对分布，结果表明低分子量肝素的分子量分布存在很大差异。这种差异归因于与未分级肝素制备方法的不同。
