Tang J S, Chao C F, Au M K
Department of Orthopaedic Surgery, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan, Republic of China.
Clin Orthop Relat Res. 1994 Mar(300):254-8.
The traditional in vitro cell culture methods provide bone cells on matrix-coated Petri dishes to grow the cells in a monolayer. This limits the usefulness in situations where there is a need to suspend anchorage dependent cells. To promote the massive production of the cultured cells and to enable the suspension of the cells in a medium, microcarrier cell culture was developed and evaluated. Isolated bone cells from rat calvaria were incubated in a microcarrier culture flask with Cytodex 1. The microcarrier cell morphology was examined by a phase contrast microscope, a scanning electron microscope, and a transmission electron microscope. Cell growth, enzyme markers, and biosynthetic characteristics were examined and compared for microcarrier and monolayer methods. The results indicate that all the characteristics of the bone cells, whether cultured in the Petri dish or microcarrier, were the same. Therefore, the studies of the function and behavior of bone cells still remain useful using the microcarrier system culture.
传统的体外细胞培养方法是在基质包被的培养皿上培养骨细胞，使细胞呈单层生长。这在需要悬浮依赖贴壁生长的细胞的情况下，实用性有限。为了促进培养细胞的大量生产并使细胞能够悬浮在培养基中，人们开发并评估了微载体细胞培养技术。从大鼠颅骨分离的骨细胞在含有Cytodex 1的微载体培养瓶中孵育。通过相差显微镜、扫描电子显微镜和透射电子显微镜观察微载体上的细胞形态。对微载体培养法和单层培养法的细胞生长、酶标记物和生物合成特性进行了检测和比较。结果表明，无论在培养皿中还是在微载体上培养，骨细胞的所有特性都是相同的。因此，使用微载体系统培养来研究骨细胞的功能和行为仍然是有用的。
