Tone Y, Miyata A, Hara S, Yukawa S, Tanabe T
Department of Pharmacology, National Cardiovascular Center Research Institute, Osaka, Japan.
FEBS Lett. 1994 Mar 7;340(3):241-4. doi: 10.1016/0014-5793(94)80146-0.
The cloned cDNA for rat thromboxane (TX) synthase with a size of 1851 bp contained a 1599-bp open reading frame which encoded a 533-amino acid protein sharing 79.7% identity with human TX synthase. RNA blot analysis was carried out with rat cells and tissues. Rat peritoneal macrophages most abundantly expressed mRNA for TX synthase, and its level was not changed by in vivo stimulation of casein. Bone marrow, spleen, lung and thymus also expressed the TX synthase gene. These findings suggest the possibility that TXA2 plays a role in the immune system.
大小为1851 bp的大鼠血栓素（TX）合酶的克隆cDNA包含一个1599 bp的开放阅读框，该阅读框编码一种533个氨基酸的蛋白质，与人TX合酶的同一性为79.7%。用大鼠细胞和组织进行RNA印迹分析。大鼠腹膜巨噬细胞最丰富地表达TX合酶的mRNA，其水平在体内酪蛋白刺激后没有改变。骨髓、脾脏、肺和胸腺也表达TX合酶基因。这些发现提示TXA2在免疫系统中发挥作用的可能性。
