Yoshihara S, Oya T, Furuya T, Goto N
Kyushu Branch Laboratory, National Institute of Animal Health, Kagoshima, Japan.
J Helminthol. 1993 Dec;67(4):279-86. doi: 10.1017/s0022149x00013274.
The Enzyme-linked immunosorbent assay (ELISA) was used to detect Ascaris suum antibodies in swine sera with adult body fluid (ABF) as an antigen. The assay was standardized with respect to the antigen concentration and serum and conjugate dilutions. Cross reaction was found between the antigen and the sera from the swine infected with Metastrongylus apri. The ELISA was more sensitive than the complement fixation test. Five protein peaks were obtained from ABF by gel filtration on Sephacryle S-300. Fraction 1 was the most specific. A 105 kDa protein in the fraction reacted with swine IgG in the serum of infected animals in Western blot analysis.
采用酶联免疫吸附测定（ELISA），以成虫体液（ABF）为抗原检测猪血清中的猪蛔虫抗体。该测定针对抗原浓度、血清和结合物稀释进行了标准化。发现该抗原与感染长刺后圆线虫的猪血清之间存在交叉反应。ELISA比补体结合试验更敏感。通过在Sephacryle S - 300上进行凝胶过滤从ABF中获得了五个蛋白峰。组分1最具特异性。在蛋白质印迹分析中，该组分中的一种105 kDa蛋白与感染动物血清中的猪IgG发生反应。
