Hardy S J, Ferrante A, Robinson B S, Johnson D W, Poulos A, Clark K J, Murray A W
Department of Immunology and University of Adelaide, Australia.
J Neurochem. 1994 Apr;62(4):1546-51. doi: 10.1046/j.1471-4159.1994.62041546.x.
A variety of fatty acids including the cis-polyunsaturated very-long-chain fatty acids (VLCFA) (> 22 carbon atoms) common in retina, spermatozoa, and brain were examined for their ability to activate protein kinase C (PKC) purified from rat brain. Arachidonic [20:4(n-6)], eicosapentaenoic [20:5(n-3)], and docosahexaenoic [22:6(n-3)] acids as well as the VLCFA dotriacontatetraenoic [32:4(n-6)] and tetratriacontahexaenoic [34:6(n-3)] were equally capable of activating PKC in vitro with maximal activity being between 25 and 50 microM. The phorbol ester 12-O-tetradecanoylphorbol 13-acetate further enhanced the in vitro activation of PKC when added to the protein kinase assay system with the fatty acids. The fully saturated arachidic acid (20:0) was inactive in both assay systems. The potential significance of the in vitro activation of PKC by the VLCFA is discussed.
研究了多种脂肪酸，包括视网膜、精子和大脑中常见的顺式多不饱和超长链脂肪酸（VLCFA，碳原子数>22）激活从大鼠脑中纯化的蛋白激酶C（PKC）的能力。花生四烯酸[20:4(n-6)]、二十碳五烯酸[20:5(n-3)]、二十二碳六烯酸[22:6(n-3)]以及VLCFA三十二碳四烯酸[32:4(n-6)]和三十四碳六烯酸[34:6(n-3)]在体外均能同等程度地激活PKC，最大活性在25至50微摩尔之间。当将佛波酯12-O-十四酰佛波醇13-乙酸酯添加到含有脂肪酸的蛋白激酶检测系统中时，可进一步增强PKC的体外激活。完全饱和的花生酸（20:0）在这两种检测系统中均无活性。讨论了VLCFA在体外激活PKC的潜在意义。
