Rahilly M A, Fleming S
Department of Pathology, University of Edinburgh, U.K.
J Pathol. 1993 Jul;170(3):297-303. doi: 10.1002/path.1711700313.
Members of the beta 1 integrin family are present at the basolateral membrane of human renal tubular epithelium in vivo and at the ventral surfaces of cultured renal epithelial cells, at the sites appropriate for cell substratum adhesion. In this study we have proven that these molecules are indeed functional in mediating cell substratum attachment in normal human renal epithelium by using monoclonal antibodies to integrin alpha subunits to block initial cell attachment. The importance of arginine-glycine-aspartic acid (RGD) recognition by cell surface receptors in various extracellular ligands was also examined using synthetic peptides. RGDS peptide strongly inhibited attachment to plain plastic or fibronectin-coated substrata but had no effect on cell adhesion to laminin-coated coverslips.
β1整合素家族成员在体内存在于人类肾小管上皮细胞的基底外侧膜，在培养的肾上皮细胞腹侧表面，位于适合细胞与基质黏附的部位。在本研究中，我们通过使用针对整合素α亚基的单克隆抗体来阻断细胞的初始黏附，证实了这些分子在介导正常人肾上皮细胞与基质附着方面确实具有功能。还使用合成肽研究了细胞表面受体对各种细胞外配体中精氨酸-甘氨酸-天冬氨酸（RGD）识别的重要性。RGDS肽强烈抑制细胞附着于普通塑料或纤连蛋白包被的基质，但对细胞黏附于层粘连蛋白包被的盖玻片没有影响。
