Kajihara H, Totović V, Gedigk P
Virchows Arch B Cell Pathol. 1975 Nov 21;19(3):221-37.
Wistar rats were injected intravenously with cod liver oil emulsion. The phagocytosis of these lipids by the Kupffer cells as well as the formation of lipid-containing lysosomes were studied electron microscopically within a period of 5 min to 12 hrs. The emulsified lipids are incorporated into the cytoplasm by membrane-vesiculation. The lipid-containing phagocytic vacuoles (lipophagosomes) were transformed into secondary lysosomes (lipophagolysosomes). This change occurs in two phases. Immediately after phagocytosis the lipophagosomes are provided with lysosomal enzymes by fusion with pre-existent lysosomes (dense bodies) of the Kupffer cells. These are mainly the so-called small homogenous dense bodies which are probably primary lysosomes of the type of "storage granules". In the second phase which begins 15-30 min after the injection; the fusion of lipophagosomes with newly produced primary lysosomes within the Golgi apparatus is predominant. Therefore, the enzyme supply of the phagocytic vacuoles does not occur at once but in small amounts during a longer period. The lipids slowly change into an amorphous electron-dense mass 12 hrs after the injection. Obviously, these changes are the first step to the formation of ceroid pigment.
给Wistar大鼠静脉注射鱼肝油乳剂。在5分钟至12小时的时间段内，通过电子显微镜研究了库普弗细胞对这些脂质的吞噬作用以及含脂质溶酶体的形成。乳化脂质通过膜泡形成被纳入细胞质。含脂质的吞噬泡（脂质吞噬体）转变为次级溶酶体（脂质吞噬溶酶体）。这种变化分两个阶段发生。吞噬作用后立即通过与库普弗细胞预先存在的溶酶体（致密体）融合，脂质吞噬体获得溶酶体酶。这些主要是所谓的小均质致密体，可能是“储存颗粒”类型的初级溶酶体。在注射后15 - 30分钟开始的第二阶段，脂质吞噬体与高尔基体中新产生的初级溶酶体的融合占主导。因此，吞噬泡的酶供应不是一次性发生，而是在较长时间内少量供应。注射后12小时，脂质缓慢转变为无定形电子致密物质。显然，这些变化是类脂色素形成的第一步。
