大鼠心脏中β-内啡肽和α-促黑素相关肽的特性研究

Characterization of beta-endorphin- and alpha-MSH-related peptides in rat heart.

作者信息

Millington W R, Evans V R, Forman L J, Battie C N

机构信息

School of Biological Sciences, University of Missouri-Kansas City 64108.

出版信息

Peptides. 1993 Nov-Dec;14(6):1141-7. doi: 10.1016/0196-9781(93)90167-f.

DOI:10.1016/0196-9781(93)90167-f

PMID:8134295

Abstract

POMC-derived peptides and mRNA have been identified in heart tissue, although POMC processing has not been fully characterized. In the present study, we found that beta-lipotropin and ACTH were localized in rat heart, although they were almost entirely converted to beta-endorphin- and alpha-MSH-related peptides. Ion exchange HPLC analysis revealed that beta-endorphin(1-31) was further processed to alpha-N-acetyl-beta-endorphin(1-31), which comprised 35.9 +/- 0.1% of total immunoreactivity, and smaller amounts of beta-endorphin(1-27), beta-endorphin(1-26), and their alpha-N-acetylated derivates. The predominant alpha-MSH immunoreactive peptides coeluted with alpha-MSH and N,O-diacetyl-alpha-MSH by reverse-phase HPLC, although small amounts of ACTH(1-13)-NH2 were also present. Thus, multiple forms of beta-endorphin and alpha-MSH are localized in rat heart. beta-Endorphin(1-31) is a minor constituent, however, indicating that nonopioid beta-endorphin peptides predominate.

摘要

在心脏组织中已鉴定出源自阿片促黑激素原（POMC）的肽和信使核糖核酸（mRNA），尽管POMC的加工过程尚未完全明确。在本研究中，我们发现β-促脂解素和促肾上腺皮质激素（ACTH）定位于大鼠心脏，尽管它们几乎完全转化为与β-内啡肽和α-促黑素（α-MSH）相关的肽。离子交换高效液相色谱（HPLC）分析显示，β-内啡肽（1-31）进一步加工为α-N-乙酰基-β-内啡肽（1-31），其占总免疫反应性的35.9±0.1%，还有少量的β-内啡肽（1-27）、β-内啡肽（1-26）及其α-N-乙酰化衍生物。通过反相HPLC分析，主要的α-MSH免疫反应性肽与α-MSH和N,O-二乙酰基-α-MSH共洗脱，尽管也存在少量的促肾上腺皮质激素（1-13）-NH2。因此，多种形式的β-内啡肽和α-MSH定位于大鼠心脏。然而，β-内啡肽（1-31）是次要成分，这表明非阿片类β-内啡肽肽占主导地位。