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参与胆酸转运至大鼠肝脏肝细胞的蛋白质的纯化及部分序列分析

Purification and partial sequence of proteins involved in the cholic acid transport into rat liver hepatocytes.

作者信息

Hoffmann A, Müllner S, Ziegler K, Fasold H

机构信息

Biozentrum Frankfurt, Abt. Biochemie, N 210/220, Germany.

出版信息

J Protein Chem. 1993 Dec;12(6):765-9. doi: 10.1007/BF01024935.

DOI:10.1007/BF01024935
PMID:8136027
Abstract

Two proteins, in previous work labeled by affinity markers derived from taurocholic acid, were purified and partially sequenced. Antibodies were raised against purified proteins, and cross-reactions were carefully checked. The influence of these antibodies upon taurocholic acid import into vesicles from rat liver plasma membranes was measured, and showed a distinct inhibition of transport in the case of the 54 kD protein.

摘要

在之前的工作中，两种由牛磺胆酸衍生的亲和标记物标记的蛋白质被纯化并进行了部分测序。针对纯化后的蛋白质制备了抗体，并仔细检查了交叉反应。测定了这些抗体对牛磺胆酸从大鼠肝细胞膜转运至囊泡的影响，结果显示，对于54 kD的蛋白质，其转运受到明显抑制。

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J Protein Chem. 1993 Dec;12(6):765-9. doi: 10.1007/BF01024935.
2
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3
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本文引用的文献

1
Characterization of the bile acid transport system in normal and transformed hepatocytes. Photoaffinity labeling of the taurocholate carrier protein.正常和转化肝细胞中胆汁酸转运系统的特性。牛磺胆酸盐载体蛋白的光亲和标记。
J Biol Chem. 1983 Jul 25;258(14):8896-901.
2
Isolation of microgram quantities of proteins from polyacrylamide gels for amino acid sequence analysis.从聚丙烯酰胺凝胶中分离微克量蛋白质用于氨基酸序列分析。
Methods Enzymol. 1983;91:227-36. doi: 10.1016/s0076-6879(83)91019-4.
3
A method for the quantitative recovery of protein in dilute solution in the presence of detergents and lipids.
一种在存在去污剂和脂质的情况下定量回收稀溶液中蛋白质的方法。
Anal Biochem. 1984 Apr;138(1):141-3. doi: 10.1016/0003-2697(84)90782-6.
4
3'-Isothiocyanatobenzamido[3H]cholate, a new affinity label for hepatocellular membrane proteins responsible for the uptake of both bile acids and phalloidin.3'-异硫氰酸苯甲酰胺基[3H]胆酸盐，一种用于肝细胞膜蛋白的新型亲和标记物，该蛋白负责胆汁酸和鬼笔环肽的摄取。
Biochim Biophys Acta. 1984 Jun 13;773(1):11-22. doi: 10.1016/0005-2736(84)90545-5.
5
Immunization, isolation of immunoglobulins, estimation of antibody titre.免疫接种、免疫球蛋白的分离、抗体滴度的测定。
Scand J Immunol Suppl. 1973;1:161-4. doi: 10.1111/j.1365-3083.1973.tb03798.x.
6
Structural analysis and specific expression of microsomal cytochrome P-450(M-1) mRNA in male rat livers.雄性大鼠肝脏微粒体细胞色素P-450（M-1）mRNA的结构分析及特异性表达
J Biol Chem. 1987 Feb 5;262(4):1706-11.
7
Evidence for glycoprotein components of the hepatocellular bile acid transporter.肝细胞胆汁酸转运体糖蛋白成分的证据。
Biochim Biophys Acta. 1987 Oct 16;903(3):510-8. doi: 10.1016/0005-2736(87)90058-7.
8
Bile acid binding proteins in hepatocellular membranes of newborn and adult rats. Identification of transport proteins with azidobenzamidotauro[14C]cholate ([14C]ABATC).新生大鼠和成年大鼠肝细胞膜中的胆汁酸结合蛋白。用叠氮苯甲酰胺牛磺胆酸[14C]盐（[14C]ABATC）鉴定转运蛋白。
Biochim Biophys Acta. 1989 Apr 14;980(2):161-8. doi: 10.1016/0005-2736(89)90395-7.
9
Two-dimensional gel electrophoresis of membrane proteins.膜蛋白的二维凝胶电泳
Biochemistry. 1976 Feb 10;15(3):616-23. doi: 10.1021/bi00648a026.
10
Effect of Na on bile acid uptake by isolated rat hepatocytes. Evidence for a heterogeneous system.钠对离体大鼠肝细胞摄取胆汁酸的影响。关于异质性系统的证据。
Hoppe Seylers Z Physiol Chem. 1978 Feb;359(2):181-92.