Costello R, Wheeler D, Orban L, Chrambach A
National Institutes of Health, Bethesda, MD 20892-0001.
Biotechniques. 1994 Jan;16(1):108-13.
Agarose gel electrophoretic separation of a lambda/HindIII DNA marker containing detectable fragments of 23 to 2 kb was carried out in the conventional submarine apparatus and in the horizontal gel slab apparatus of Wieme, using identical samples, agarose, gel width and procedure for ethidium bromide staining. In the Wieme apparatus, the gel on its microscope slide support is immersed in an immiscible solvent such as petroleum ether or silicone oil. Although band resolution and speed of migration are equivalent between gels run in the two systems, the relative fluorescence intensity of the ethidium bromide-stained bands is substantially more responsive to an increase in DNA length in the Wieme gels than in the submarine gels. The predicted relative fluorescence is by an average factor of 0.5 less than that observed after electrophoresis in the Wieme apparatus but is by an average factor of 3.4 more than that observed on bands derived from the submarine technique.
使用相同的样品、琼脂糖、凝胶宽度以及溴化乙锭染色程序，在传统的潜水式装置和维姆水平凝胶板装置中，对含有23至2kb可检测片段的λ/HindIII DNA标记物进行琼脂糖凝胶电泳分离。在维姆装置中，置于显微镜载玻片支架上的凝胶被浸入不混溶的溶剂中，如石油醚或硅油。尽管在两个系统中运行的凝胶之间的条带分辨率和迁移速度相当，但与潜水式凝胶相比，维姆凝胶中溴化乙锭染色条带的相对荧光强度对DNA长度增加的响应要大得多。预测的相对荧光比在维姆装置中电泳后观察到的平均低0.5倍，但比潜水技术获得的条带观察到的平均高3.4倍。
