Steuerwald N, Lambert H, Steinleitner A J, Herrera R J
San Francisco Center for Reproductive Medicine, CA 94118.
Biotechniques. 1994 Jan;16(1):82-4.
Alphoid repeat sequences on the X and Y chromosomes were successfully and consistently coamplified to determine the sex of single cells. Buccal cells served as a convenient model for single blastomeres obtained from preimplantation embryos through in vitro fertilization. The potential use of this technique for preimplantation genetic diagnosis is discussed.
成功且一致地共扩增了X和Y染色体上的α卫星重复序列，以确定单细胞的性别。颊细胞作为通过体外受精从植入前胚胎获得的单个卵裂球的便捷模型。讨论了该技术在植入前基因诊断中的潜在应用。
