Mushiake K, Motoyoshi F, Kondo N, Orii T
Department of Pediatrics, Gifu University School of Medicine, Japan.
Exp Clin Immunogenet. 1993;10(4):189-94.
Three patients with common variable immunodeficiency (CVID) were analyzed for translation from mu mRNA by the cell-free translation method and for expression of mu mRNA by northern blotting. In cases 1 and 2, the mu chain was not detected in the products by cell-free translation nor was mu mRNA detected by northern blotting. In case 3, mu mRNA was detected at a low level. This suggests that the disorders occur between rearrangement of the Ig genes and transcription of the C mu gene in cases 1 and 2, and that the functions of rearrangement and transcription are qualitatively preserved to a certain degree in case 3. Thus, the pathogeneses of CVID are variable and more research on regulatory mechanisms of B cell development is necessary to understand each case of CVID.
采用无细胞翻译方法分析了3例普通变异型免疫缺陷（CVID）患者的μ mRNA翻译情况，并通过Northern印迹法分析了μ mRNA的表达。在病例1和病例2中，无细胞翻译产物中未检测到μ链，Northern印迹法也未检测到μ mRNA。在病例3中，检测到低水平的μ mRNA。这表明在病例1和病例2中，疾病发生在Ig基因重排和Cμ基因转录之间，而在病例3中，重排和转录功能在一定程度上在质量上得以保留。因此，CVID的发病机制是多样的，需要对B细胞发育的调控机制进行更多研究，以了解每一例CVID。
