Dassouli A, Manin M, Veyssiere G, Jean C
CNRS URA 360, Université Blaise Pascal, Les Cézeaux, Aubière, France.
J Steroid Biochem Mol Biol. 1994 Jan;48(1):121-8. doi: 10.1016/0960-0760(94)90258-5.
Mouse vas deferens protein (MVDP), a member of the aldo-keto reductase superfamily, is exclusively produced in the vas deferens. To better understand androgen-regulated MVDP gene expression we have used RNA hybridization to study the effects of androgens on the steady-state levels of MVDP mRNA in vas deferens epithelial cell subcultures. Northern blot analysis revealed that these cells only express MVDP mRNA in the presence of androgens. There was a close relationship between MVDP mRNA levels and dihydrotestosterone concentrations. MVDP mRNA is induced over a period of 24h and maximal induction is about 25-fold. Treatment of cells with cycloheximide completely abolished the observed androgen effect suggesting that the induction of the MVDP gene by androgens depends on continuous protein synthesis. Transient transfection of vas deferens epithelial cells with MMTV-CAT vector showed that these cells contained functional androgen receptors and that they are a suitable system to study androgen effect on MVDP gene regulatory elements.
小鼠输精管蛋白（MVDP）是醛糖还原酶超家族的成员之一，仅在输精管中产生。为了更好地理解雄激素对MVDP基因表达的调控，我们利用RNA杂交技术研究了雄激素对输精管上皮细胞亚培养物中MVDP mRNA稳态水平的影响。Northern印迹分析显示，这些细胞仅在雄激素存在的情况下表达MVDP mRNA。MVDP mRNA水平与二氢睾酮浓度之间存在密切关系。MVDP mRNA在24小时内被诱导，最大诱导倍数约为25倍。用环己酰亚胺处理细胞完全消除了观察到的雄激素效应，这表明雄激素对MVDP基因的诱导依赖于持续的蛋白质合成。用MMTV-CAT载体瞬时转染输精管上皮细胞表明，这些细胞含有功能性雄激素受体，并且它们是研究雄激素对MVDP基因调控元件影响的合适系统。
