Casavilla F A, Selby R, Abu-Elmagd K, Reyes J, Nakamura K, Wright H I, Tzakis A, Todo S, Demetris A J, Fung J J
Pittsburgh Transplantation Institute, PA.
Clin Transplant. 1994 Feb;8(1):49-53.
Our procedure for donor harvesting and preserving intestinal grafts has matured. In 27 consecutive cases, a protocol was established whose essentials consist of (a) selecting hemodynamically stable donors, (b) antibiotic pretreatment of the donor, and (c) short warm ischemic times (< 40 minutes). Assessment of graft quality can be achieved by daily inspection of stomas, inspection for diarrhea > 2.5 1/day in adults or > 300 ml in children, and weekly protocol or clinically directed endoscopic biopsies. Edema and microscopic separation of the mucosal surface and sloughing are routinely found during the first few post-engraftment days, but the crypt cells remain and regenerate a normal mucosa within a week. Recovery of a normal mucosal surface took place in all cases.
我们获取和保存供体肠道移植物的程序已经成熟。在连续27例病例中，制定了一项方案，其要点包括：（a）选择血流动力学稳定的供体；（b）对供体进行抗生素预处理；（c）缩短热缺血时间（<40分钟）。通过每日检查造口、检查成人腹泻量>2.5升/天或儿童腹泻量>300毫升，以及每周进行方案规定的或临床指导下的内镜活检，可以评估移植物质量。在移植后的最初几天，通常会发现黏膜表面出现水肿、微观分离和脱落，但隐窝细胞仍然存在，并在一周内再生出正常黏膜。所有病例均恢复了正常的黏膜表面。
