Jayabaskaran C, Puttaraju M
Department of Biochemistry, Indian Institute of Science Bangalore.
Biochem Mol Biol Int. 1993 Dec;31(5):983-95.
Total cytoplasmic tRNAs from cucumber hypocotyls were fractionated by two-dimensional polyacrylamide gel electrophoresis into about 56 species. Of these, 32 tRNA species were identified by aminoacylation as tRNA specific for 15 amino acids. Furthermore, cytoplasmic tRNA(Phe) and tRNA(Leu(NAA)) were purified to homogeneity by the combination of RPC-5 column chromatography and two-dimensional polyacrylamide gel electrophoresis. The primary structure of tRNA(Leu(NAA)) and the modified nucleotides of tRNA(Phe) have been determined. The sequence of cucumber cytoplasmic tRNA(Leu(NAA)) is identical to that of bean cytoplasmic tRNA(Leu(NAA)) except for three base differences in the variable loop.
黄瓜下胚轴的总细胞质tRNA通过二维聚丙烯酰胺凝胶电泳分离成约56种。其中，32种tRNA通过氨酰化被鉴定为15种氨基酸特异的tRNA。此外，通过RPC-5柱色谱和二维聚丙烯酰胺凝胶电泳相结合的方法，将细胞质tRNA（Phe）和tRNA（Leu（NAA））纯化至同质。已确定tRNA（Leu（NAA））的一级结构和tRNA（Phe）的修饰核苷酸。黄瓜细胞质tRNA（Leu（NAA））的序列与菜豆细胞质tRNA（Leu（NAA））的序列相同，只是可变环中有三个碱基差异。
