Frankel A, Tsao M S, Viallet J
Department of Medicine, Montreal General Hospital, Quebec, Canada.
Cancer Res. 1994 Apr 1;54(7):1613-6.
We detected low level expression of the gastrin-releasing peptide and neuromedin-B receptor mRNAs in cultures of human bronchial epithelium from 4 of 6 individuals. Bombesin receptor subtype-3 mRNA was undetectable in these cells. An elevation of intracellular calcium concentration was observed in response to bradykinin (6 of 6) and neurotensin (1 of 5) but not to bombesin (0 of 6), vasopressin (0 of 6), or cholecystokinin (0 of 3). In contrast, such responses are frequently noted in lung cancer cell lines. Bombesin did not stimulate the in vitro growth of an immortalized human bronchial epithelium cell line expressing low levels of bombesin receptor mRNAs. We conclude that bombesin receptors are expressed at low levels in human bronchial epithelium cells which may acquire greater responsiveness to multiple neuropeptides in the course of multistep carcinogenesis.
我们在6名个体中4人的人支气管上皮细胞培养物中检测到胃泌素释放肽和神经降压素B受体mRNA的低水平表达。在这些细胞中未检测到蛙皮素受体亚型-3 mRNA。观察到细胞内钙浓度升高是对缓激肽（6/6）和神经降压素（1/5）的反应，但对蛙皮素（0/6）、血管加压素（0/6）或胆囊收缩素（0/3）无反应。相比之下，在肺癌细胞系中经常观察到这种反应。蛙皮素不会刺激表达低水平蛙皮素受体mRNA的永生化人支气管上皮细胞系的体外生长。我们得出结论，蛙皮素受体在人支气管上皮细胞中低水平表达，在多步骤致癌过程中可能对多种神经肽获得更高的反应性。
