蛙肝高分子量（140 kDa）酸性磷酸酶碳水化合物链类型的表征
Characterization of the type of carbohydrate chains of the higher molecular weight (140 kDa) acid phosphatase of the frog liver.
作者信息
Kubicz A, Szalewicz A
机构信息
Section on Macromolecular Analysis, National Institute of Health and Human Development, Bethesda, MD 20892.
出版信息
Int J Biochem. 1993 Dec;25(12):1957-61. doi: 10.1016/0020-711x(88)90331-x.
PMID:8138034
Abstract
- The higher molecular weight, (HMW, M(r) 140 kDa) acid phosphatase (AcPase) of the frog liver (Rana esculenta) was separated into enzymatically active components by isoelectric focusing in an immobilized pH gradient and their carbohydrate chains were analyzed by specific lectin binding after native blotting. 2. The lectin-binding patterns obtained with ConA, WGA, LcH and PNA as well as with WGA and PNA after desialylation indicate that the frog liver HMW AcPase contains predominantly N-linked complex and/or hybride type carbohydrate chains with terminal sialic acid and fucose residues; O-glycosylated enzyme components with free and sialic acid substituted Gal-GalNAc sequences were also detected.
摘要
- 通过在固定化pH梯度中进行等电聚焦，将青蛙肝脏（食用蛙）的高分子量（HMW，分子量140 kDa）酸性磷酸酶（AcPase）分离为具有酶活性的组分，并在天然印迹后通过特异性凝集素结合分析其糖链。2. 用伴刀豆球蛋白A（ConA）、小麦胚凝集素（WGA）、荆豆凝集素（LcH）和花生凝集素（PNA）以及去唾液酸化后用WGA和PNA获得的凝集素结合模式表明，青蛙肝脏HMW AcPase主要含有具有末端唾液酸和岩藻糖残基的N-连接的复杂型和/或杂合型糖链；还检测到具有游离和唾液酸取代的Gal-GalNAc序列的O-糖基化酶组分。
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