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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Thøgersen V B, Heickendorff L, Ledet T

Research Laboratory of Biochemical Pathology, Aarhus Kommunehospital, University of Aarhus, Denmark.

Int J Biochem. 1994 Jan;26(1):55-9. doi: 10.1016/0020-711x(94)90195-3.

Sulphate labelled proteoglycans (PG) synthesized by cultured human arterial smooth muscle cell have been quantified using an improved method based on a combination of specific enzymes and ethanol precipitation. 2. The present method gives quantitative data of PGs and subclasses allowing batchwise analysis of a large number of samples. 3. Approximately 81% +/- 1.7% (mean +/- SD, n = 6) of total PGs synthesized by human arterial smooth muscle cells accumulated in medium. 4. In cell layer and medium chondroitin sulphate proteoglycan constituted 65.0% +/- 0.3% and 75.8% +/- 0.7% (mean +/- SD, n = 3), respectively of sulphated PGs. 5. Heparan sulphate proteoglycan accounted for 26.8% +/- 0.6% in cell layer and 22.6% +/- 0.5% (mean +/- SD, n = 3) in medium of sulphated PGs.

采用一种基于特定酶与乙醇沉淀相结合的改进方法，对培养的人动脉平滑肌细胞合成的硫酸盐标记蛋白聚糖（PG）进行了定量分析。2. 本方法可提供PG及其亚类的定量数据，从而能够对大量样本进行批量分析。3. 人动脉平滑肌细胞合成的总PG中，约81%±1.7%（平均值±标准差，n = 6）积聚在培养基中。4. 在细胞层和培养基中，硫酸软骨素蛋白聚糖分别占硫酸化PG的65.0%±0.3%和75.8%±0.7%（平均值±标准差，n = 3）。5. 硫酸乙酰肝素蛋白聚糖在细胞层中占硫酸化PG的26.8%±0.6%，在培养基中占22.6%±0.5%（平均值±标准差，n = 3）。

Thøgersen V B, Heickendorff L, Ledet T

Research Laboratory of Biochemical Pathology, Aarhus Kommunehospital, University of Aarhus, Denmark.

Int J Biochem. 1994 Jan;26(1):55-9. doi: 10.1016/0020-711x(94)90195-3.

Sulphate labelled proteoglycans (PG) synthesized by cultured human arterial smooth muscle cell have been quantified using an improved method based on a combination of specific enzymes and ethanol precipitation. 2. The present method gives quantitative data of PGs and subclasses allowing batchwise analysis of a large number of samples. 3. Approximately 81% +/- 1.7% (mean +/- SD, n = 6) of total PGs synthesized by human arterial smooth muscle cells accumulated in medium. 4. In cell layer and medium chondroitin sulphate proteoglycan constituted 65.0% +/- 0.3% and 75.8% +/- 0.7% (mean +/- SD, n = 3), respectively of sulphated PGs. 5. Heparan sulphate proteoglycan accounted for 26.8% +/- 0.6% in cell layer and 22.6% +/- 0.5% (mean +/- SD, n = 3) in medium of sulphated PGs.

采用一种基于特定酶与乙醇沉淀相结合的改进方法，对培养的人动脉平滑肌细胞合成的硫酸盐标记蛋白聚糖（PG）进行了定量分析。2. 本方法可提供PG及其亚类的定量数据，从而能够对大量样本进行批量分析。3. 人动脉平滑肌细胞合成的总PG中，约81%±1.7%（平均值±标准差，n = 6）积聚在培养基中。4. 在细胞层和培养基中，硫酸软骨素蛋白聚糖分别占硫酸化PG的65.0%±0.3%和75.8%±0.7%（平均值±标准差，n = 3）。5. 硫酸乙酰肝素蛋白聚糖在细胞层中占硫酸化PG的26.8%±0.6%，在培养基中占22.6%±0.5%（平均值±标准差，n = 3）。