Tertrin-Clary C, De La Llosa P
C R Acad Hebd Seances Acad Sci D. 1975 Sep 22;281(12):823-6.
A comparative study was made of the stimulation of rat ovary adenyl cyclase by ovine luteinizing hormone (LH) and its methylated drrivative (obtained by reductive alkylation of the lysine residues). The apparent Km (half maximal activity) of native LH and methylated LH was similar but the maximal activity obtained with the methylated derivative was significantly higher (1.2-1.8 times). When EDTA was added to the incubation medium the maximal activity obtained with the methylated LH diminished and became even lower than that obtained with native LH.
对绵羊促黄体生成素（LH）及其甲基化衍生物（通过赖氨酸残基的还原烷基化获得）刺激大鼠卵巢腺苷酸环化酶的情况进行了比较研究。天然LH和甲基化LH的表观Km（半最大活性）相似，但甲基化衍生物获得的最大活性显著更高（1.2 - 1.8倍）。当向孵育培养基中加入EDTA时，甲基化LH获得的最大活性降低，甚至低于天然LH获得的最大活性。
