用于小鼠单链抗体可变区文库构建的引物序列优化 - Suppr

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Optimization of primer sequences for mouse scFv repertoire display library construction.

作者信息

Zhou H, Fisher R J, Papas T S

机构信息

Laboratory of Cellular Biochemistry, PRI/DynCorp, Frederick, MD 21702.

出版信息

Nucleic Acids Res. 1994 Mar 11;22(5):888-9. doi: 10.1093/nar/22.5.888.

DOI:10.1093/nar/22.5.888

PMID:8139934

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC307900/

Abstract

摘要

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/15db/307900/052122863ea7/nar00029-0198-a.jpg

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本文引用的文献

Protein engineering of antibody binding sites: recovery of specific activity in an anti-digoxin single-chain Fv analogue produced in Escherichia coli.抗体结合位点的蛋白质工程：在大肠杆菌中产生的抗地高辛单链Fv类似物中恢复比活性。

Proc Natl Acad Sci U S A. 1988 Aug;85(16):5879-83. doi: 10.1073/pnas.85.16.5879.

Generation of a large combinatorial library of the immunoglobulin repertoire in phage lambda.利用λ噬菌体构建免疫球蛋白全套的大型组合文库。

Science. 1989 Dec 8;246(4935):1275-81. doi: 10.1126/science.2531466.

Isoforms of the human ets-1 protein: generation by alternative splicing and differential phosphorylation.人类ets-1蛋白的异构体：通过可变剪接和差异磷酸化产生

Oncogene. 1990 May;5(5):675-81.

Multi-subunit proteins on the surface of filamentous phage: methodologies for displaying antibody (Fab) heavy and light chains.

Nucleic Acids Res. 1991 Aug 11;19(15):4133-7. doi: 10.1093/nar/19.15.4133.

By-passing immunization. Human antibodies from V-gene libraries displayed on phage.绕过免疫接种。噬菌体展示的来自V基因文库的人抗体。

J Mol Biol. 1991 Dec 5;222(3):581-97. doi: 10.1016/0022-2836(91)90498-u.

Monoclonal antibodies specific to human ETS-2 oncoprotein: recognition of epitopes clustered on the B domain.

Hybridoma. 1990 Dec;9(6):559-71. doi: 10.1089/hyb.1990.9.559.

By-passing immunization: building high affinity human antibodies by chain shuffling.

Biotechnology (N Y). 1992 Jul;10(7):779-83. doi: 10.1038/nbt0792-779.

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