Strom R, Ravagnan G, Salfi V
Eur J Biochem. 1976 Feb 2;62(1):95-101. doi: 10.1111/j.1432-1033.1976.tb10101.x.
Upon preincubation with halogenated isoxazolyl-penicillins, the activity of Bacillus cereus beta-lactamase is decreased by a factor proportional to the inhibitor concentration. This inactivation, which concerns essentially the catalytic rate constant of the enzyme, occurs only at or above 37 degrees C with cloxacillin and dicloxacillin, and even at 25 degrees C with flucloxacillin. The dependence of the inactivation rate upon inhibitor concentration is consistent, in the former case, with the occurrence of a bimolecular reaction, and in the latter case, with a bimolecular process followed by a monomolecular step.
在与卤代异恶唑基青霉素预孵育后，蜡样芽孢杆菌β-内酰胺酶的活性会以与抑制剂浓度成比例的系数降低。这种失活主要涉及酶的催化速率常数，仅在37℃及以上时，与氯唑西林和双氯西林发生失活，而与氟氯西林反应时，甚至在25℃时就会发生失活。在前一种情况下，失活速率对抑制剂浓度的依赖性与双分子反应的发生一致，而在后一种情况下，则与双分子过程后接单分子步骤一致。
