Honrath U, Matsuda Y, Sonnenberg H
Department of Physiology, University of Toronto, Ontario, Canada.
Regul Pept. 1994 Jan 13;49(3):211-6. doi: 10.1016/0167-0115(94)90143-0.
A selective antagonist for the cGMP-linked ANF receptor was used to assess inhibition of cardiovascular and renal actions of atrial natriuretic factor (ANF). Two groups of anesthetized rats were injected with antagonist or vehicle, respectively, prior to an infusion of ANF. A third group received neither antagonist injection nor ANF infusion and served as a time control. Compared to ANF infusion alone, prior antagonist administration was associated with significant reduction of both the hypotension and hemoconcentration following peptide infusion, although significant residual effects were still present. Glomerular filtration rates during ANF infusion were significantly lower in the antagonist group. The increases in urinary salt and water excretion were also partially blocked by the antagonist. Microcatheterization studies showed significant partial reversal of ANF-induced inhibition of sodium chloride and water reabsorption in the medullary collecting duct. We conclude that the antagonist is an effective specific blocker of the cardiovascular, renal hemodynamic, and tubular effects of ANF, providing a useful new tool to elucidate the regulatory roles of this peptide hormone system.
一种与环磷酸鸟苷（cGMP）相关的心房钠尿肽（ANF）受体选择性拮抗剂被用于评估对心房钠尿肽心血管和肾脏作用的抑制情况。两组麻醉大鼠在输注ANF前分别注射拮抗剂或赋形剂。第三组既未注射拮抗剂也未输注ANF，作为时间对照。与单独输注ANF相比，预先给予拮抗剂与肽输注后低血压和血液浓缩的显著降低相关，尽管仍存在显著的残余效应。拮抗剂组在输注ANF期间的肾小球滤过率显著更低。拮抗剂也部分阻断了尿盐和水排泄的增加。微导管插入研究显示，ANF诱导的髓质集合管氯化钠和水重吸收抑制有显著的部分逆转。我们得出结论，该拮抗剂是ANF心血管、肾脏血流动力学和肾小管效应的有效特异性阻滞剂，为阐明这种肽类激素系统的调节作用提供了一种有用的新工具。
