Pereira C A, Lucchiari M A, Modolell M, Kuhn L, Lefkovits I
Instituto Butantan, Laboratorio de Immunologia Viral, Sao Paulo, Brazil.
Res Virol. 1993 Nov-Dec;144(6):479-86. doi: 10.1016/s0923-2516(06)80063-4.
The activation of bone-marrow-derived macrophages by IFN-gamma (IFN gamma) partially inhibits mouse hepatitis virus 3 (MHV3) replication only in cells from resistant A/J mice, and not in cells originating from susceptible BALB/c mice. The computer image analysis of gels obtained from 2D-SDS-PAGE of extracted proteins of IFN gamma-activated A/J or BALB/c macrophages enabled us to identify and tag several gene products that were synthesized at elevated or diminished levels. Comparisons of the patterns of non-activated and IFN gamma-activated A/J macrophages revealed 3 gene products which increased, 1 which newly appeared, 6 which decreased and 20 which disappeared upon IFN gamma activation. The protein pattern of BALB/c macrophages revealed 13 gene products which increased, 8 which decreased and 8 which disappeared in IFN gamma-activated BALB/c macrophages. Whether these proteins are involved in the induction of an antiviral state against MHV3 growth remains to be investigated. Macrophages from mice with different genetic background (A/J and BALB/c), upon IFN gamma activation, behave differently at a molecular level, and this observation is consistent with their distinct expression of antiviral state against MHV3.
γ干扰素（IFNγ）激活骨髓来源的巨噬细胞，仅在来自抗性A/J小鼠的细胞中部分抑制小鼠肝炎病毒3型（MHV3）的复制，而在源自易感BALB/c小鼠的细胞中则无此作用。对从经IFNγ激活的A/J或BALB/c巨噬细胞提取的蛋白质进行二维SDS-PAGE凝胶的计算机图像分析，使我们能够识别并标记几种表达水平升高或降低的基因产物。比较未激活和经IFNγ激活的A/J巨噬细胞的模式，发现有3种基因产物增加，1种新出现，6种减少，20种在IFNγ激活后消失。BALB/c巨噬细胞的蛋白质模式显示，在经IFNγ激活的BALB/c巨噬细胞中有13种基因产物增加，8种减少，8种消失。这些蛋白质是否参与诱导针对MHV3生长的抗病毒状态仍有待研究。来自不同遗传背景（A/J和BALB/c）小鼠的巨噬细胞在IFNγ激活后，在分子水平上表现不同，这一观察结果与它们对MHV3抗病毒状态的不同表达一致。
